Article
Isolation and characterization of Aspergillus oryzae vacuolar protein sorting mutants.
Applied and environmental microbiology - 1 Aug 2005
Ohneda Mamoru, Arioka Manabu, Kitamoto Katsuhiko
Abstract excerpt
The vacuolar protein sorting (vps) system in the filamentous fungus Aspergillus oryzae, which has unique cell polarity and the ability to secrete large amounts of proteins, was evaluated by using mutants that missort vacuolar proteins into the medium. Vacuolar carboxypeptidase Y (CPY) fused with enhanced green fluorescent protein (EGFP) was used as a vacuolar marker. Twenty dfc (dim EGFP fluorescence in conidia)...
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