A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast.

doi:10.1083/jcb.128.5.779

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Article

A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast.

The Journal of cell biology - 1 Mar 1995

Vida T A, Emr S D

Abstract excerpt

We have used a lipophilic styryl dye, N-(3-triethylammoniumpropyl)-4- (p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM 4-64), as a vital stain to follow bulk membrane-internalization and transport to the vacuole in yeast. After treatment for 60 min at 30 degrees C, FM 4-64 stained the...

Topics

Biological Transport
Cell-Free System
Endocytosis
Energy Metabolism
Fungal Proteins
Microscopy
Mitochondria
Mutation
Pyridinium Compounds
Quaternary Ammonium Compounds
Saccharomyces cerevisiae
Staining and Labeling
Vacuoles

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