Article
Characterization of plasminogen activator inhibitor 1 mutants containing the P13 to P10 region of ovalbumin or antithrombin III: evidence that the P13 residue contributes significantly to the active to substrate transition.
Biochimica et biophysica acta - 8 Sept 1998
Gils A, Vleugels N, Tobback K, Knockaert I, Declerck P J
Abstract excerpt
The serpin plasminogen activator inhibitor 1 (PAI-1) can occur, in vitro, in both an inhibitory and a non-inhibitory but cleavable substrate form. In the present study, we have evaluated the effect of replacing the P13 to P10 region of PAI-1 (Val-Ala-Ser-Ser), with the P13 to P10 region of either...
Topics
- Antithrombin III
- Enzyme Stability
- Kinetics
- Mutation
- Ovalbumin
- Plasminogen Activator Inhibitor 1
- Protein Conformation
- Recombinant Fusion Proteins
- Serine Endopeptidases
- Serine Proteinase Inhibitors
- Tissue Plasminogen Activator
- Urokinase-Type Plasminogen Activator