Article
GTP-dependent conformational changes associated with the functional switch between Galpha and cross-linking activities in brain-derived tissue transglutaminase.
Journal of molecular biology - 2 Oct 1998
Monsonego A, Friedmann I, Shani Y, Eisenstein M, Schwartz M
Abstract excerpt
GTP and Ca2+, two well-known modulators of intracellular signaling pathways, control a structural/functional switch between two vital and mutually exclusive activities, cross-linking and Galpha activity, in the same enzyme. The enzyme, a brain-derived tissue-type transglutaminase (TGase), was rec...
Topics
- Animals
- Binding Sites
- Brain
- Calcium
- Cell Line
- Cross-Linking Reagents
- GTP Phosphohydrolases
- GTP-Binding Proteins
- Guanosine Triphosphate
- Humans
- Kinetics
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Precipitin Tests
- Protein Binding
- Protein Conformation
- Protein Glutamine gamma Glutamyltransferase 2