Article
Differential chemical probing of a group II self-splicing intron identifies bases involved in tertiary interactions and supports an alternative secondary structure model of domain V.
RNA (New York, N.Y.) - 1 Sept 1998
Costa M, Christian E L, Michel F
Abstract excerpt
Dimethyl sulfate modification was used to probe for tertiary structural elements in the group II intron PI.LSU/2 from the mitochondrial pre-ribosomal RNA of the brown alga Pylaiella littoralis. Modification of the lariat form of the intron under conditions that allow both native folding and confo...
Topics
- Alkylating Agents
- Base Sequence
- DNA Primers
- Introns
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Phaeophyceae
- Polymerase Chain Reaction
- RNA Precursors
- RNA Splicing
- RNA, Small Nuclear
- Spliceosomes
- Sulfuric Acid Esters