Anchoring antibodies to membranes using a diphtheria toxin T domain-ZZ fusion protein as a pH sensitive membrane anchor.

doi:10.1016/s0014-5793(98)00890-4

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Article

Anchoring antibodies to membranes using a diphtheria toxin T domain-ZZ fusion protein as a pH sensitive membrane anchor.

FEBS letters - 14 Aug 1998

Nizard P, Liger D, Gaillard C, Gillet D

Abstract excerpt

We have constructed a fusion protein, T-ZZ, in which the IgG-Fc binding protein ZZ was fused to the C-terminus of the diphtheria toxin transmembrane domain (T domain). While soluble at neutral pH, T-ZZ retained the capacity of the T domain to bind to phospholipid membranes at acidic pH. Once anch...

Topics

Animals
Antibodies, Monoclonal
Cell Membrane Permeability
Diphtheria Toxin
Escherichia coli
Hydrogen-Ion Concentration
Immunoglobulin G
Liposomes
Mice
Mutation
Rabbits
Recombinant Fusion Proteins
Staphylococcal Protein A

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