Article
Effects of mutations in proline 345 on insertion of diphtheria toxin into model membranes.
The Journal of membrane biology - 15 Jan 1999
Zhan H, Elliott J L, Shen W H, Huynh P D, Finkelstein A, Collier R J
Abstract excerpt
Translocation of the catalytic domain of diphtheria toxin (DT) across the endosomal membrane to the cytoplasm of mammalian cells requires the low-pH-dependent insertion of a hydrophobic helical hairpin (TH8-TH9) that is buried within the T domain of the native protein. Mutations of Pro345, which terminates helix TH8, have been reported to block toxicity for Vero cells. We found that mutant toxins in which Pro345...
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