Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli.

doi:10.1126/science.8259521

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Article

Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli.

Science (New York, N.Y.) - 10 Dec 1993

Derman A I, Prinz W A, Belin D, Beckwith J

Abstract excerpt

Disulfide bonds are rarely found in cytoplasmic proteins. Mutations were selected for in Escherichia coli that allow disulfide bond formation in the cytoplasm. In the presence of these mutations, export-defective versions of alkaline phosphatase and mouse urokinase were able to fold into their en...

Topics

Alkaline Phosphatase
Cysteine
Cytoplasm
Disulfides
Escherichia coli
Genes, Bacterial
Mutation
Oxidation-Reduction
Protein Folding
Protein Sorting Signals
Recombinant Proteins
Thioredoxin-Disulfide Reductase
Urokinase-Type Plasminogen Activator

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