Article
A point mutation in the human cytomegalovirus DNA polymerase gene selected in vitro by cidofovir confers a slow replication phenotype in cell culture.
Virology - 1 Sept 1998
Cihlar T, Fuller M D, Mulato A S, Cherrington J M
Abstract excerpt
In cell culture, cidofovir (CDV) was used to select a human cytomegalovirus (HCMV) strain with decreased drug susceptibility. The genotypic characterization of this virus revealed a single base substitution resulting in a K513N amino acid alteration in the viral DNA polymerase (UL54). Performed i...
Topics
- Amino Acid Sequence
- Antiviral Agents
- Cells, Cultured
- Cidofovir
- Cytomegalovirus
- Cytosine
- DNA Replication
- DNA, Viral
- DNA-Directed DNA Polymerase
- Drug Resistance, Microbial
- Enzyme Repression
- Exonucleases
- Ganciclovir
- Molecular Sequence Data
- Organophosphonates
- Organophosphorus Compounds
- Phenotype
- Phosphodiesterase I