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Article

Cold adaptation of a psychrophilic chitinase: a mutagenesis study.

Protein engineering - 1 Jul 2003

Mavromatis K, Feller G, Kokkinidis M, Bouriotis V

Abstract excerpt

The gene encoding chitinase ArChiB from the Antarctic Arthrobacter sp. TAD20 has been expressed in Escherichia coli and the recombinant enzyme purified to homogeneity. In an effort to engineer cold-adapted biocatalysts through rational redesign to operate at elevated temperatures, we performed several mutations aiming to increase the rigidity of the molecular edifice of the selected psychrophilic chitinase. The...

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Identifiers

PMID: 12915727
DOI: 10.1093/protein/gzg069

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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