Article
Cold adaptation of a psychrophilic chitinase: a mutagenesis study.
Protein engineering - 1 Jul 2003
Mavromatis K, Feller G, Kokkinidis M, Bouriotis V
Abstract excerpt
The gene encoding chitinase ArChiB from the Antarctic Arthrobacter sp. TAD20 has been expressed in Escherichia coli and the recombinant enzyme purified to homogeneity. In an effort to engineer cold-adapted biocatalysts through rational redesign to operate at elevated temperatures, we performed se...
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