Article
Restriction endonuclease-mediated selective polymerase chain reaction: a novel assay for the detection of K-ras mutations in clinical samples.
The American journal of pathology - 1 Aug 1998
Ward R, Hawkins N, O'Grady R, Sheehan C, O'Connor T, Impey H, Roberts N, Fuery C, Todd A
Abstract excerpt
The enriched polymerase chain reaction (PCR) assay has been used extensively in the detection of ras gene mutations in many types of human malignancies. Although it is very sensitive, it has a number of features that limit its use in the routine diagnostic laboratory. The aim of this study was to...
Topics
- Adenocarcinoma
- Adult
- Aged
- Aged, 80 and over
- Breast Neoplasms
- Colorectal Neoplasms
- Deoxyribonucleases, Type II Site-Specific
- Female
- Genes, ras
- Humans
- Kidney Neoplasms
- Male
- Middle Aged
- Mutation
- Pancreatic Neoplasms