Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Restriction endonuclease-mediated selective polymerase chain reaction: a novel assay for the detection of K-ras mutations in clinical samples.

The American journal of pathology - 1 Aug 1998

Ward R, Hawkins N, O'Grady R, Sheehan C, O'Connor T, Impey H, Roberts N, Fuery C, Todd A

Abstract excerpt

The enriched polymerase chain reaction (PCR) assay has been used extensively in the detection of ras gene mutations in many types of human malignancies. Although it is very sensitive, it has a number of features that limit its use in the routine diagnostic laboratory. The aim of this study was to develop a novel enriched PCR strategy, in which the concurrent activity of the restriction enzyme BstNI and Taq...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adenocarcinoma
Share to Aged

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Aged, 80 and over

Share to Breast Neoplasms

Share to Colorectal Neoplasms

Share to Genes, ras

Share to Mutation

Share to Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 9708798
DOI: 10.1016/S0002-9440(10)65581-2
PMCID: PMC1852993

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Restriction endonuclease-mediated selective polymerase chain reaction: a novel assay for the detection of K-ras mutations in clinical samples.PMID 9708798Read ↗

Detection of rare mutant alleles by restriction endonuclease-mediated selective-PCR: assay design and optimization.Semantic proximity Read ↗

Quantitative enriched PCR (QEPCR), a highly sensitive method for detection of K-ras oncogene mutation.Semantic proximity Read ↗

Rapid and sensitive nonradioactive detection of mutant K-ras genes via 'enriched' PCR amplification.Semantic proximity Read ↗

Sensitive detection of trace amounts of KRAS codon 12 mutations by a fast and novel one-step technique.Semantic proximity Read ↗

Selective 'stencil'-aided pre-PCR cleavage of wild-type sequences as a novel approach to detection of mutant K-RAS.Semantic proximity Read ↗

Detection of mutant K-ras DNA in plasma or serum of patients with colorectal cancer.Semantic proximity Read ↗

Restriction endonuclease-catalyzed cleavage of DNA stimulates subsequent polymerase chain reaction and enables discrimination between normal and mutant K-RAS alleles.Semantic proximity Read ↗

Noninvasive detection through REMS-PCR technique of K-ras mutations in stool DNA of patients with colorectal cancer.Semantic proximity Read ↗

Detection of codon 61 point mutations of the K-ras gene in lung and colorectal cancers by enriched PCR.Semantic proximity Read ↗

Mutant-enriched PCR and allele-specific hybridization reaction to detect K-ras mutations in stool DNA: high prevalence in a large sample of older adults.Semantic proximity Read ↗

K-ras oncogene activation in adenocarcinoma of the human pancreas. A study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele-specific oligonucleotide hybridization.Semantic proximity Read ↗

Enrichment polymerase chain reaction for the detection of Ki-ras mutations: relevance of Taq polymerase error rate, initial DNA copy number, and reaction conditions on the emergence of false-positive mutant bands.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.