Article
Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis.
Nucleic acids research - 15 Mar 1998
Guldberg P, Grønbak K, Aggerholm A, Platz A, thor Straten P, Ahrenkiel V, Hokland P, Zeuthen J
Abstract excerpt
Denaturing gradient gel electrophoresis (DGGE) in combination with PCR and 'GC-clamping' has proven highly efficient as a method for detection of DNA sequence differences. Due to strand dissociation phenomena, however, its use has been limited to the analysis of sequences with a relatively low co...
Topics
- Base Composition
- Base Sequence
- DNA
- DNA Mutational Analysis
- DNA Primers
- Electrophoresis
- Evaluation Studies as Topic
- Genes, p16
- Humans
- Mutation
- Nucleic Acid Denaturation
- Polymerase Chain Reaction
- Sulfites