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Article

Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis.

Nucleic acids research - 15 Mar 1998

Guldberg P, Grønbak K, Aggerholm A, Platz A, thor Straten P, Ahrenkiel V, Hokland P, Zeuthen J

Abstract excerpt

Denaturing gradient gel electrophoresis (DGGE) in combination with PCR and 'GC-clamping' has proven highly efficient as a method for detection of DNA sequence differences. Due to strand dissociation phenomena, however, its use has been limited to the analysis of sequences with a relatively low content of GC pairs. This paper describes how treatment of template DNA with sodium bisulphite drastically lowers the...

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Identifiers

PMID: 9490806
DOI: 10.1093/nar/26.6.1548
PMCID: PMC147432

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