Article
Analysis of sequence variations in several human genes using phosphoramidite bond DNA fragmentation and chip-based MALDI-TOF.
Genome research - 1 Jan 2004
Smylie Kevin J, Cantor Charles R, Denissenko Mikhail F
Abstract excerpt
The challenge in the postgenome era is to measure sequence variations over large genomic regions in numerous patient samples. This massive amount of work can only be completed if more accurate, cost-effective, and high-throughput solutions become available. Here we describe a novel DNA fragmentation approach for single nucleotide polymorphism (SNP) discovery and sequence validation. The base-specific cleavage is...
Topics
- 3' Untranslated Regions
- Base Sequence
- Carrier Proteins
- Cholesterol Ester Transfer Proteins
- DNA
- DNA Fragmentation
- Genes
- Genetic Variation
- Genotype
- Glycoproteins
- Humans
- I-kappa B Proteins
- Ion Channels
- Membrane Transport Proteins