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Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA

1 Mar 1993

Abstract excerpt

A transformation scheme for Cryptococcus neoformans to yield high-frequency, integrative events was developed. Adenine auxotrophs from a clinical isolate of C. neoformans serotype A were complemented by the cryptococcal phosphoribosylaminoimidazole carboxylase gene (ade2) with a biolistic DNA delivery system. Comparison of two DNA delivery systems (electroporation versus a biolistic system) showed notable...

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Identifiers

PMID: 8444802
DOI: 10.1128/jb.175.5.1405-1411.1993

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