Article
Chain-terminating mutations in the APC gene lead to alterations in APC RNA and protein concentration.
Genes, chromosomes & cancer - 1 Aug 1998
Gismondi V, Stagnaro P, Pedemonte S, Biticchi R, Presciuttini S, Grammatico P, Sala P, Bertario L, Groden J, Varesco L
Abstract excerpt
APC mutations introduce premature stop codons into the open reading frame of the gene, leading to the formation of truncated tumor suppressor proteins. Both RNA and protein levels are likely to be profoundly altered by such nonsense mutations. To test this hypothesis, Western blotting and RT-PCR...
Topics
- Adenomatous Polyposis Coli Protein
- Adolescent
- Adult
- Alternative Splicing
- Cell Line, Transformed
- Cytoskeletal Proteins
- Genes, APC
- Humans
- Middle Aged
- Mutation
- Peptide Chain Termination, Translational
- RNA, Neoplasm
- Tumor Cells, Cultured