Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.

doi:10.3892/ijo.13.2.269

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Article

Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.

International journal of oncology - 1 Aug 1998

Lipinski L J, Ross H L, Zajc B, Sayer J M, Jerina D M, Dipple A

Abstract excerpt

Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by placing each of the four isomeric benzo[a]pyrene diol epoxide-deoxyguanosine adducts at the 13th nucleotide from the 3'-end of two different sequence contexts. Using an 11-m...

Topics

7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
DNA Adducts
DNA Polymerase I
DNA Replication
Deoxyguanosine
Mutation
Oligonucleotides
Templates, Genetic

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