Article
Efficiency and accuracy of SOS-induced DNA polymerases replicating benzo[a]pyrene-7,8-diol 9,10-epoxide A and G adducts.
The Journal of biological chemistry - 15 Feb 2002
Shen Xuan, Sayer Jane M, Kroth Heiko, Ponten Ingrid, O'Donnell Mike, Woodgate Roger, Jerina Donald M, Goodman Myron F
Abstract excerpt
Nucleotide incorporation fidelity, mismatch extension, and translesion DNA synthesis efficiencies were determined using SOS-induced Escherichia coli DNA polymerases (pol) II, IV, and V to copy 10R and 10S isomers of trans-opened benzo[a]pyrene-7,8-diol 9,10-epoxide (BaP DE) A and G adducts. A-BaP DE adducts were bypassed by pol V with moderate accuracy and considerably higher efficiency than by pol II or IV....
Topics
- 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
- Base Pair Mismatch
- DNA Adducts
- DNA Repair
- DNA-Directed DNA Polymerase
- Kinetics
- Models, Chemical
- Mutagens
- Mutation
- SOS Response, Genetics