Affinity purification of yeast cytochrome oxidase with biotinylated subunits 4, 5, or 6.

doi:10.1006/abio.1998.2683

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Article

Affinity purification of yeast cytochrome oxidase with biotinylated subunits 4, 5, or 6.

Analytical biochemistry - 15 Jun 1998

Glerum D M, Tzagoloff A

Abstract excerpt

Null mutants in COX4, COX5a, or COX6, which encode subunits 4, 5, and 6 of yeast cytochrome oxidase are blocked in assembly of the enzyme. The mutants are complemented by gene constructs expressing cytochrome oxidase subunits with a carboxyl terminal extension containing a biotinylation signal se...

Topics

Alleles
Avidin
Biotinylation
Chromatography, Affinity
Electron Transport Complex IV
Fungal Proteins
Mitochondria
Polymerase Chain Reaction
Recombinant Fusion Proteins
Saccharomyces cerevisiae

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