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Article

Rapid purification of wildtype and mutant cytochrome c oxidase from Rhodobacter sphaeroides by Ni(2+)-NTA affinity chromatography.

FEBS letters - 10 Jul 1995

Mitchell D M, Gennis R B

Abstract excerpt

A rapid and highly efficient method of purifying the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been developed. This method relies upon a six-histidine affinity tag fused to the C-terminus of subunit I, which confers to the oxidase a high affinity for Ni(2+)-nitrilotriacetic acid (NTA) agarose. The histidine-tagged oxidase can be purified rapidly and with high yield by one affinity...

Topics

Amino Acid Sequence
Base Sequence
Chromatography, Affinity
Electron Transport Complex IV
Histidine
Molecular Sequence Data
Mutation
Nitrilotriacetic Acid
Oligodeoxyribonucleotides
Organometallic Compounds
Rhodobacter sphaeroides

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Identifiers

PMID: 7615070
DOI: 10.1016/0014-5793(95)00626-k

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7615070-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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