Article
Identification and characterization of novel low-temperature-inducible promoters of Escherichia coli.
Journal of bacteriology - 1 Dec 1992
Qoronfleh M W, Debouck C, Keller J
Abstract excerpt
Escherichia coli promoters that are more active at low temperature (15 to 20 degrees C) than at 37 degrees C were identified by using the transposon Tn5-lac to generate promoter fusions expressing beta-galactosidase (beta-Gal). Tn5-lac insertions that resulted in low-temperature-regulated beta-Gal expression were isolated by selecting kanamycin-resistant mutants capable of growth on lactose minimal medium at 15...
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