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Article

Identification and characterization of novel low-temperature-inducible promoters of Escherichia coli.

Journal of bacteriology - 1 Dec 1992

Qoronfleh M W, Debouck C, Keller J

Abstract excerpt

Escherichia coli promoters that are more active at low temperature (15 to 20 degrees C) than at 37 degrees C were identified by using the transposon Tn5-lac to generate promoter fusions expressing beta-galactosidase (beta-Gal). Tn5-lac insertions that resulted in low-temperature-regulated beta-Gal expression were isolated by selecting kanamycin-resistant mutants capable of growth on lactose minimal medium at 15...

Topics

Anti-Bacterial Agents
Base Sequence
Blotting, Northern
Blotting, Southern
Cloning, Molecular
DNA Transposable Elements
DNA, Bacterial
Escherichia coli
Molecular Sequence Data
Mutation
Promoter Regions, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Restriction Mapping

Temperature

beta-Galactosidase

Source

Open in PubMed Open full text

Identifiers

PMID: 1334067
DOI: 10.1128/jb.174.24.7902-7909.1992
PMCID: PMC207524

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1334067-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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