Article
Transcriptional attenuation and differential mRNA stability in the regulation of the Escherichia coli melibiose operon.
Journal of biochemistry - 1 Jun 1994
Shimamoto T, Noguchi K, Kuroda M, Tsuda M, Tsuchiya T
Abstract excerpt
The organization of the melibiose operon of Escherichia coli is promoter-melA-melB. The amount of the product (alpha-galactosidase) of the first gene (melA) is much larger than that of the product (melibiose permease) of the second gene (melB). Using the chloramphenicol acetyltransferase gene (cat) as reporter, we found that there was an element between melA and melB, which reduced the expression of the...
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