Article
The generation of human gammadelta T cell repertoires during fetal development.
Journal of immunology (Baltimore, Md. : 1950) - 15 Jun 1998
McVay L D, Jaswal S S, Kennedy C, Hayday A, Carding S R
Abstract excerpt
The nature of how human gammadelta T cells are normally generated is not clear. We have used an RT-PCR assay and DNA sequencing to identify and compare delta-encoded TCRs (TCRDs) that are generated de novo in the fetal gut, liver, and thymus and to determine when, where, and how the TCRD repertoi...
Topics
- Animals
- Antibodies, Monoclonal
- Digestive System
- Embryonic and Fetal Development
- Flow Cytometry
- Gene Expression Regulation, Developmental
- Gene Rearrangement, delta-Chain T-Cell Antigen Receptor
- Humans
- Mice
- Peptide Mapping
- Phenotype
- Polymerase Chain Reaction
- Receptors, Antigen, T-Cell, gamma-delta
- Thymus Gland
- Transcription, Genetic