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Article

Purification and characterization of the active-site-mutated recombinant human mu-calpain expressed in baculovirus-infected insect cells.

Biochemical and biophysical research communications - 29 May 1998

Hitomi K, Uchiyama Y, Ohkubo I, Kunimatsu M, Sasaki M, Maki M

Abstract excerpt

Recombinant human mu-calpain whose active site Cys-115 was substituted with Ser was expressed in insect cells using baculovirus system. The mutant mu-calpain, purified using an affinity-column of calpastatin oligopeptides, had no proteolytic activities of autolysis and caseinolysis. The large sub...

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Source

Identifiers

PMID: 9618272
DOI: 10.1006/bbrc.1998.8686

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-9618272-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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