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Article

Overexpression, purification, and characterization of human m-calpain and its active site mutant, m-C105S-calpain, using a baculovirus expression system.

Journal of biochemistry - 1 Nov 1998

Masumoto H, Yoshizawa T, Sorimachi H, Nishino T, Ishiura S, Suzuki K

Abstract excerpt

Recombinant human m-calpain was produced in a soluble form at a level of 20 mg/liter of Sf-9 cell culture by the coexpression of recombinant human m-calpain large (m80K) and small (30K) subunits using a baculovirus expression system. The expressed m-calpain was purified by sequential column chromatographies on DEAE-Toyopearl, gel-filtration, and Mono Q by the same method used to purify native m-calpain. The...

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PMID: 9792919
DOI: 10.1093/oxfordjournals.jbchem.a022213

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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