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Article

Affinity purification of human m-calpain through an intrinsically disordered inhibitor, calpastatin.

PloS one - 1 Jan 2017

Nguyen Hung Huy, Varadi Mihaly, Tompa Peter, Pauwels Kris

Abstract excerpt

Calpains are calcium-activated proteases that have biomedical and biotechnological potential. Their activity is tightly regulated by their endogenous inhibitor, calpastatin that binds to the enzyme only in the presence of calcium. Conventional approaches to purify calpain comprise multiple chromatographic steps, and are labor-intensive, leading to low yields. Here we report a new purification procedure for the...

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Share to Biotinylation
Share to Calpain
Share to Chromatography, Affinity

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Chromatography, Ion Exchange

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Share to Sequence Analysis, Protein

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Identifiers

PMID: 28319173
DOI: 10.1371/journal.pone.0174125
PMCID: PMC5358782

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