Article
The importance of conserved amino acid residues in p94 protease sub-domain IIb and the IS2 region for constitutive autolysis.
FEBS letters - 5 Mar 2008
Ono Yasuko, Hayashi Chikako, Doi Naoko, Tagami Mai, Sorimachi Hiroyuki
Abstract excerpt
p94/calpain 3, a skeletal muscle-specific member of calpain protease family, is characterized by apparent Ca(2+)-independence during exhaustive autolysis and concomitant proteolysis of non-self substrates. The purpose of our study was to comprehensively profile the structural basis of p94 enabling activation in the cytosol without an extra Ca(2+). Ca(2+)-dependent p94 mutants were screened using "p94-trapping",...
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