Using 2-step PCR and restriction endonuclease digestion to detect K-ras mutation in paraffin-embedded tissues: is it reliable?

Abstract excerpt

In this study, we detected the existence of both wild type and mutant K-ras DNA on the cutting board and in the solutions for tissue processing in our pathology laboratory. The detection rate of K-ras mutant DNA inversely correlated with the frequency of the solution changed, implying that DNA contaminant including K-ras mutant may be accumulated while increasing specimens were processed. The exact routes of...

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