Article
Method for the purification of tissue DNA suitable for PCR after fixation with Bouin's fluid. Uses and limitations in microsatellite typing.
Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Jun 1997
Longy M, Duboue B, Soubeyran P, Moynet D
Abstract excerpt
Paraffin-embedded tissues are often the only available material to perform polymerase chain reaction (PCR)-based analysis in various medical purposes. Unfortunately, the use in many countries of acid fixatives such as Bouin's fluid limits the use of such a material for molecular analysis. This ar...
Topics
- Acetic Acid
- Alleles
- Chromosome Aberrations
- Cryopreservation
- DNA, Neoplasm
- Electrophoresis, Agar Gel
- Fixatives
- Formaldehyde
- Humans
- Microsatellite Repeats
- Neoplasms
- Paraffin Embedding
- Picrates
- Polymerase Chain Reaction
- Tissue Fixation