Article
Method for the purification of tissue DNA suitable for PCR after fixation with Bouin's fluid. Uses and limitations in microsatellite typing.
Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Jun 1997
Longy M, Duboue B, Soubeyran P, Moynet D
Abstract excerpt
Paraffin-embedded tissues are often the only available material to perform polymerase chain reaction (PCR)-based analysis in various medical purposes. Unfortunately, the use in many countries of acid fixatives such as Bouin's fluid limits the use of such a material for molecular analysis. This article reports the methodological details of a DNA purification technique from Bouin-fixed and paraffin-embedded samples...
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