Article
Evaluation of a DNA Extraction and Purification Protocol Using Archived Formalin-fixed Paraffin-embedded Tissues for BRAF Mutations Analysis in Papillary Thyroid Microcarcinomas.
Applied immunohistochemistry & molecular morphology : AIMM - 1 Jan 2019
Nechifor-Boilă Adela, Loghin Andrada, Descotes Françoise, Decaussin-Petrucci Myriam, Borda Angela
Abstract excerpt
The isolation of good quality genomic DNA from formalin-fixed, paraffin-embedded tissues is challenging, especially in cases of small tissue specimens. The aim of our study was to evaluate a DNA extraction protocol using formalin-fixed, paraffin-embedded tissues in our laboratory and apply this method to a series of papillary thyroid microcarcinomas (PTMCs). A total of 25 PTMCs and 3 papillary thyroid carcinoma...
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