Article
The role of the pro-sequence in the processing and secretion of the thermolysin-like neutral protease from Bacillus cereus.
Molecular microbiology - 1 Jun 1992
Wetmore D R, Wong S L, Roche R S
Abstract excerpt
The Bacillus cereus cnp gene coding for the thermolysin-like neutral protease (TNP) has been cloned, sequenced, and expressed in Bacillus subtilis. The protease is first produced as a pre-pro-protein (M(r) = 61,000); the pro-peptide is approximately two-thirds of the size of the mature protein. The pro-sequence has been compared with those of six other TNPs, and significant homologies have been found....
Topics
- Amino Acid Sequence
- Bacillus cereus
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- Enzyme Precursors
- Metalloendopeptidases
- Molecular Sequence Data
- Mutation
- Protein Processing, Post-Translational
- Sequence Homology, Nucleic Acid