Article
The genetic diversity of Mycobacterium tuberculosis strains in Thailand studied by amplification of DNA segments containing direct repetitive sequences.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease - 1 Feb 1998
Namwat W, Luangsuk P, Palittapongarnpim P
Abstract excerpt
OBJECTIVE: To develop and use a polymerase chain reaction (PCR) method for studying the genetic diversity of Mycobacterium tuberculosis. DESIGN: Two polymorphic DNA segments of M. tuberculosis H37Rv were identified and sequenced. Primers were then designed for simultaneous amplification of both polymorphic segments. The method was used for studying 179 clinical isolates of M. tuberculosis that had been previously...
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