Article
Production of TNFalpha and IL-6 by activated whole blood from HIV-1 infected patients detected by a one-stage procedure: relationship with the phenotype of HIV-1 isolates.
Microbiology and immunology - 1 Jan 1997
Benyoucef S, Hober D, Shen L, Ajana F, De Groote D, Bocket-Mouton L, Gérard Y, Lion G, Vilain V, Wattré P
Abstract excerpt
Diluted whole blood (WB) culturing may be the most appropriate milieu in which to study cytokine production in vitro. We tested TNFalpha and IL-6 production using small volumes of WB (25 microl) from HIV-1 positive patients with a one-step procedure that combines WB stimulation with LPS, PHA and...
Topics
- Blood Cells
- CD4 Lymphocyte Count
- CD4-Positive T-Lymphocytes
- Cell Culture Techniques
- Coculture Techniques
- Cytopathogenic Effect, Viral
- Female
- HIV Infections
- HIV-1
- HeLa Cells
- Heparin
- Humans
- Immunoenzyme Techniques
- Interleukin-6