Article
Further investigation of the light chain shifting phenomenon: light chain replacement through secondary rearrangement induced by lectin stimulation in the hybridoma cell line HB4C5.
Cytotechnology - 1 Jan 1997
Krungkasem C, Ueda K, Tachibana H, Shirahata S
Abstract excerpt
We found that when the hybridoma cell line HB4C5 was stimulated with wheat germ agglutinin (WGA), loss of production of the original lambda light chain occurred, followed by production of new light chain, which mirrored the reaction when stimulated with concanavalin A (ConA). We previously report...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Membrane
- DNA-Binding Proteins
- Gene Expression
- Gene Rearrangement, B-Lymphocyte, Light Chain
- Genetic Variation
- Homeodomain Proteins
- Humans
- Hybridomas
- Immunoglobulin Class Switching
- Immunoglobulin lambda-Chains
- Molecular Sequence Data
- Nuclear Proteins