Article
Substrate specificity of native and mutated cytochrome P450 (CYP102A3) from Bacillus subtilis.
Journal of biotechnology - 19 Feb 2004
Lentz Oliver, Urlacher Vlada, Schmid Rolf D
Abstract excerpt
Within the Bacillus subtilis genome sequencing project, two monooxygenases (CYP102A2 and CYP102A3) were discovered which revealed a similarity of 76% to the well-known cytochrome P450 BM-3 (CYP102A1) of Bacillus megaterium. All enzymes are natural fusion proteins consisting of a heme domain and a reductase domain. We here report the cloning, expression and characterization of B. subtilis enzyme CYP102A3. The...
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