Article
Analysis of the allele-specific PCR method for the detection of neoplastic disease.
Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Feb 1997
Rhodes C H, Honsinger C, Porter D M, Sorenson G D
Abstract excerpt
PCR assays for the presence of mutant K-ras or p53 sequences are potentially useful as sensitive tests for tumor diagnosis. The technical challenge is to design assays sensitive enough to detect a few molecules of mutant DNA yet sufficiently specific that a false positive signal is not produced b...
Topics
- Alleles
- DNA Primers
- DNA-Directed DNA Polymerase
- Genes, p53
- Genes, ras
- Humans
- Mutation
- Neoplasms
- Peptide Fragments
- Polymerase Chain Reaction
- Taq Polymerase
- Templates, Genetic