Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Analysis of the allele-specific PCR method for the detection of neoplastic disease.

Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Feb 1997

Rhodes C H, Honsinger C, Porter D M, Sorenson G D

Abstract excerpt

PCR assays for the presence of mutant K-ras or p53 sequences are potentially useful as sensitive tests for tumor diagnosis. The technical challenge is to design assays sensitive enough to detect a few molecules of mutant DNA yet sufficiently specific that a false positive signal is not produced by a 10(5)- or 10(6)-fold excess of normal DNA. We determined the detection limit of allele-specific PCR (ASA) as a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to DNA Primers
Share to Genes, p53

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genes, ras

Share to Mutation

Share to Neoplasms

Share to Peptide Fragments

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 9028737
DOI: 10.1097/00019606-199702000-00008

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Analysis of the allele-specific PCR method for the detection of neoplastic disease.PMID 9028737Read ↗

Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe.Semantic proximity Read ↗

Detection of rare K-ras codon 12 mutations using allele-specific competitive blocker PCR.Semantic proximity Read ↗

High-fidelity DNA polymerase enhances the sensitivity of a peptide nucleic acid clamp PCR assay for K-ras mutations.Semantic proximity Read ↗

Microarray-based resequencing by apyrase-mediated allele-specific extension.Semantic proximity Read ↗

Selective 'stencil'-aided pre-PCR cleavage of wild-type sequences as a novel approach to detection of mutant K-RAS.Semantic proximity Read ↗

K-ras point mutation detection in lung cancer: comparison of two approaches to somatic mutation detection using ARMS allele-specific amplification.Semantic proximity Read ↗

Quantitative determination of the ratio of mutated to normal ras genes in the blood of leukemia patients by allele-specific PCR.Semantic proximity Read ↗

A method to detect ras point mutations in small subpopulations of cells.Semantic proximity Read ↗

A nonradioactive, allele-specific polymerase chain reaction for reproducible detection of rare mutations in large amounts of genomic DNA: application to human k-ras.Semantic proximity Read ↗

A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.Semantic proximity Read ↗

Sensitive detection of p53 gene mutations by a 'mutant enriched' PCR-SSCP technique.Semantic proximity Read ↗

Molecular detection of codon 12 K-RAS mutations in circulating DNA from serum of colorectal cancer patients.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.