Article
Systematic gain-of-function genetics in Drosophila.
Development (Cambridge, England) - 1 Mar 1998
Rørth P, Szabo K, Bailey A, Laverty T, Rehm J, Rubin G M, Weigmann K, Milán M, Benes V, Ansorge W, Cohen S M
Abstract excerpt
A modular misexpression system was used to carry out systematic gain-of-function genetic screens in Drosophila. The system is based on inducible expression of genes tagged by insertion of a P-element vector carrying a GAL4-regulated promoter oriented to transcribe flanking genomic sequences. To identify genes involved in eye and wing development, the 2300 independent lines were screened for dominant phenotypes....
Topics
- Animals
- CCAAT-Enhancer-Binding Proteins
- Cell Movement
- DNA-Binding Proteins
- Drosophila
- Drosophila Proteins
- Eye
- Female
- Gene Expression Regulation, Developmental
- Genes, Dominant
- Genes, Insect
- Genome
- Male
- Mutation