Article
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Development (Cambridge, England) - 1 Jun 1993
Brand A H, Perrimon N
Abstract excerpt
We have designed a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. The gene encoding the yeast transcriptional activator GAL4 is inserted randomly into the Drosophila genome to drive GAL4 expression from one of a diverse array of genomic enhancers. It is then possible to introduce a gene containing GAL4 binding...
Topics
- Animals
- Base Sequence
- Drosophila
- Enhancer Elements, Genetic
- Eye
- Gene Expression Regulation
- Genes, Dominant
- Microscopy, Electron, Scanning
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype