Article
S49 cells endogenously express subtype 2 somatostatin receptors which couple to increase protein tyrosine phosphatase activity in membranes and down-regulate Raf-1 activity in situ.
Cellular signalling - 1 Nov 1997
Dent P, Wang Y, Gu Y Z, Wood S L, Reardon D B, Mangues R, Pellicer A, Schonbrunn A, Sturgill T W
Abstract excerpt
S49 cells expressed type 2 somatostatin receptors (sstr2) by immunoblotting. Analysis by reverse transcription and polymerase chain reaction (RT-PCR) methodologies showed that S49 cells express predominantly sstr2A and sstr2B mRNAs; other subtypes were either not detected, in the case of sstr1, sstr3, sstr4, or variably detected, in the case of sstr5. No mutations were present in S49 cells at codon 12, 13, or 61...
Topics
- Animals
- Blotting, Western
- Cell Membrane
- Down-Regulation
- GTP-Binding Proteins
- Genes, ras
- Mice
- Mutation
- Protein Tyrosine Phosphatases
- Proto-Oncogene Proteins c-raf