Article
Free luciferase may acquire a more favorable conformation than ribosome-associated luciferase for its activity expression.
FEBS letters - 17 Nov 1997
Yang F, Jing G Z, Zhou J M, Zheng Y Z
Abstract excerpt
A variant of firefly luciferase in which the C-terminal end was extended with 44 amino acid residues served as a model protein in this study. After transcription and translation in vitro, the enzyme activity was measured when still attached to the ribosome and when released from the ribosome by incubation with RNase A or puromycin. It was found that the C-terminally extended luciferase already had activity when...
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