Article
Expression and characterization of the Renilla luciferase with the cumulative mutation.
Protein expression and purification - 1 May 2018
Ishibashi Matsujiro, Kawanabe Ryo, Amaba Norie, Arai Shigeki, Laksmi Fina Amreta, Komori Kenta, Tokunaga Masao
Abstract excerpt
Luciferase from Renilla reniformis (RLuc) is a good research tool as a reporter protein and bioimaging probes, yielding blue light using the substrate coelenterazine. However, the applications are limited since RLuc is unstable under various conditions. Therefore, an attempt was made to increase RLuc thermostability. In this study, 5 mutations reported previously [1] and one mutation obtained using site-directed...
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