Article
Analysis of gene expression in the rat hippocampus using Real Time PCR reveals high inter-individual variation in mRNA expression levels.
Journal of neuroscience research - 15 Jan 2002
Alfonso Julieta, Pollevick Guido D, Castensson Anja, Jazin Elena, Frasch Alberto C C
Abstract excerpt
In mammals, gene transcription is a step subjected to tight regulation mechanisms. In fact, changes in mRNA levels in the central nervous system (CNS) can account for numerous phenotypic differences in brain function. We performed a high-resolution analysis of mRNA expression levels for 37 genes selected from a normal rat hippocampus cDNA library. mRNA amounts were quantified using a Real Time PCR SYBR Green...
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