Article
Direct repeat sequences in the Streptomyces chitinase-63 promoter direct both glucose repression and chitin induction.
Proceedings of the National Academy of Sciences of the United States of America - 25 Nov 1997
Ni X, Westpheling J
Abstract excerpt
The chi63 promoter directs glucose-sensitive, chitin-dependent transcription of a gene involved in the utilization of chitin as carbon source. Analysis of 5' and 3' deletions of the promoter region revealed that a 350-bp segment is sufficient for wild-type levels of expression and regulation. The analysis of single base changes throughout the promoter region, introduced by random and site-directed mutagenesis,...
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