Article
A mutant spacer sequence between -35 and -10 elements makes the Plac promoter hyperactive and cAMP receptor protein-independent.
Proceedings of the National Academy of Sciences of the United States of America - 4 May 2004
Liu Mofang, Tolstorukov Michael, Zhurkin Victor, Garges Susan, Adhya Sankar
Abstract excerpt
To determine whether the spacer region between the -35 and -10 elements plays any sequence-specific role, we randomized the GC-rich sequence ((-20)CCGGCTCG(-13)) within the spacer region of the cAMP-dependent lac promoter and selected an activator-independent mutant, which showed extraordinarily high intrinsic activity. The hyperactive promoter is obtained by incorporation of a specific 10-bp-long AT-rich DNA...
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