Article
Construction and application of plasmid- and transposon-based promoter-probe vectors for Streptomyces spp. that employ a Vibrio harveyi luciferase reporter cassette.
Journal of bacteriology - 1 Jan 1992
Sohaskey C D, Im H, Schauer A T
Abstract excerpt
Several versatile promoter-probe vectors have been constructed for Streptomyces strains which utilize the production of blue-green light as a measure of transcription activity. Three plasmid vectors (two high and one low copy number) and two transposons are described. The multicopy plasmids pRS11...
Topics
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Probes
- DNA Transposable Elements
- Genetic Vectors
- Luciferases
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Recombination, Genetic
- Streptomyces
- Vibrio
