Article
Protein secretion in Streptomyces griseus N2-3-11: characterization of the secA gene and its growth phase-dependent expression.
FEMS microbiology letters - 1 Nov 1997
Pöhling S, Piepersberg W, Wehmeier U F
Abstract excerpt
The chromosomal region encoding the secA gene of Streptomyces griseus N2-3-11 was cloned and analyzed. The secA gene encodes a polypeptide of 939 aa with a molecular mass of 105 kDa. The growth defect of temperature sensitive Escherichia coli secA mutants was not restored by the S. griseus SecA....
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Biological Transport, Active
- Cloning, Molecular
- DNA Primers
- DNA, Bacterial
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Developmental
- Gene Transfer Techniques
- Genes, Bacterial
- Membrane Transport Proteins
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Promoter Regions, Genetic