Article
Protein secretion in Streptomyces griseus N2-3-11: characterization of the secA gene and its growth phase-dependent expression.
FEMS microbiology letters - 1 Nov 1997
Pöhling S, Piepersberg W, Wehmeier U F
Abstract excerpt
The chromosomal region encoding the secA gene of Streptomyces griseus N2-3-11 was cloned and analyzed. The secA gene encodes a polypeptide of 939 aa with a molecular mass of 105 kDa. The growth defect of temperature sensitive Escherichia coli secA mutants was not restored by the S. griseus SecA. The secA promoter was analyzed and the transcriptional start point of the gene was determined. Northern blot and...
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