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Article

Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus.

FEMS microbiology letters - 1 Dec 2000

Jiang H, Kendrick K E

Abstract excerpt

An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, SXXK, SXN, and KTG. The pbpA gene was located downstream of a gene homologous to the Bacillus...

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Identifiers

PMID: 11094280
DOI: 10.1111/j.1574-6968.2000.tb09403.x

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