Article
Effects of replacement of promoters and modification of the leader peptide region of the amy gene of Streptomyces griseus on synthesis and secretion of alpha-amylase by Streptomyces lividans.
Molecular & general genetics : MGG - 1 Dec 1991
Vigal T, Gil J A, Daza A, García-González M D, Villadas P, Martín J F
Abstract excerpt
Five different mutations were introduced into the leader peptide region of the alpha-amylase gene of Streptomyces griseus IMRU 3570. A mutation which increased the positive charge of the N-terminal region of the leader peptide enhanced the secretion of alpha-amylase by two- to threefold. Replacement of the native promoter of the amylase gene by the promoter of the Tn5 neo gene or by the promoter of the saf gene...
Topics
- Amino Acid Sequence
- Gene Expression
- Kinetics
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Protein Sorting Signals
- Streptomyces