Article
Characterization of the secA gene of Streptomyces lividans encoding a protein translocase which complements and Escherichia coli mutant defective in the ATPase activity of SecA.
Gene - 17 Oct 1996
Blanco J, Coque J J, Martín J F
Abstract excerpt
The secA gene of Streptomyces lividans was cloned using as probe a 57-mer oligonucleotide based on conserved sequences of the Escherichia coli secA and the Bacillus subtilis div genes. It encodes a protein of 946 amino acids (aa) with a deduced M(r) of 106,079, with high similarity to all known S...
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Azides
- Bacterial Proteins
- Base Sequence
- Biological Transport
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Genetic Complementation Test
- Membrane Transport Proteins
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- SEC Translocation Channels
- SecA Proteins
- Sequence Analysis, DNA