Article
A dominant negative mutation in Saccharomyces cerevisiae methionine aminopeptidase-1 affects catalysis and interferes with the function of methionine aminopeptidase-2.
Archives of biochemistry and biophysics - 15 Nov 1997
Klinkenberg M, Ling C, Chang Y H
Abstract excerpt
Methionine aminopeptidase (MetAP) enzymes require the metal ion cobalt, but little is known about the role of cobalt in the structural stability or catalysis of these enzymes. In Escherichia coli MetAP, for which a crystal structure is available, the five amino acid residues liganding the two cob...
Topics
- Aminopeptidases
- Aspartic Acid
- Binding Sites
- Cobalt
- Escherichia coli
- Genes, Fungal
- Kinetics
- Metalloendopeptidases
- Methionyl Aminopeptidases
- Mutagenesis, Site-Directed
- Mutation
- Recombinant Proteins
- Saccharomyces cerevisiae
- Substrate Specificity