Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.

doi:10.18926/AMO/30789

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Article

Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.

Acta medica Okayama - 1 Oct 1997

Mihara K, Miyazaki M, Kondo T, Fushimi K, Tsuji T, Inoue Y, Fukaya K, Ishioka C, Namba M

Abstract excerpt

We used a yeast functional assay (functional analysis of separated alleles in yeast: FASAY) to determine the p53 gene status of human cell lines maintained in our laboratory. This assay enables the researcher to score wild-type p53 expression on the basis of the ability of expressed p53 to transactivate the reporter gene HIS 3 via the p53-responsive GAL 1 promoter in Saccharomyces cerevisiae. The cell lines...

Topics

Alleles
Carcinoma, Hepatocellular
Genes, p53
Genetic Therapy
Humans
Liver Neoplasms
Mutation
Polymerase Chain Reaction
Tumor Cells, Cultured
Yeasts

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Open in PubMed

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PMID: 9359923
DOI: 10.18926/AMO/30789

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